Conventional and molecular surveillance of paratuberculosis in Tamil Nadu

1. Conventional and molecular surveillance of
paratuberculosis in Tamil Nadu
Vinodh Kumar,O.R
Division of Epidemiology
ICAR-Indian Veterinary Research Institute
Izatnagar, Uttar Pradesh, India

5. A1 , B1- negative control
C1, D1 – positive control

6. A1, A2-
positive
control
B1, B2 –
negative
control
Gamma interferon assay (Bovigam,Biocor,USA)

7. A1 , B1- negative control
C1, D1 – positive control
Out of 1034 sera samples tested
126 (12.24 % ) were positive to
MAP IgG antibodies.
ELISA on 486 samples which
represented Chennai and sub
urban areas showed 78 were
positive (16.04%).
Seropositivity in 460 sheep
brought for slaughter at the local
abattoir showed 44 (9.56%)
were positive by ELISA

10. Districts Number of
samples
PCR Positives Percentage
Salem 220 54 24.55
Karur 37 4 10.81
Namakkal 35 6 17.14
Dharmapuri 41 5 12.20
Krishnagiri 28 4 14.29
Erode 82 15 18.29
Trichy 141 17 12.06
Perambalur 67 15 22.39
Ariyalur 25 7 28.00
Dindigul 72 8 11.11
Madurai 156 31 19.87
Thanjavur 37 4 10.81
Coimbatore 93 10 10.75
1034 180 17.41

11. Calvings Number
of
samples
ELISA Positivity
%
PCR Positivity
%
AFB Positivity
%
Heifer 32 14 43.75 21 65.63 17 53.13
First calving 87 19 21.84 24 27.59 4 4.60
Second
calving
527 57 10.82 92 17.46 38 7.21
Third calving 308 28 9.09 55 17.86 28 9.09
Fourth
calving
64 12 18.75 2 3.13 4 6.25
Fifth calving 16 9 56.25 6 37.50 0 0.00
Calvings vs percentage positivity

12. Districts Number of samples AFB positives Percentage
Salem 220 20 9.09
Karur 37 2 5.41
Namakkal 35 1 2.86
Dharmapuri 41 2 4.88
Krishnagiri 28 1 3.57
Erode 82 7 8.54
Trichy 141 12 8.51
Perambalur 67 5 7.46
Ariyalur 25 3 12.00
Dindigul 72 4 5.56
Madurai 156 12 7.69
Thanjavur 37 2 5.41
Coimbatore 93 8 8.60
1034 79 7.64

13. Districts Number of
samples
ELISA
positives
Percentage
Salem 220 40 18.18
Karur 37 2 5.41
Namakkal 35 3 8.57
Dharmapuri 41 3 7.32
Krishnagiri 28 2 7.14
Erode 82 8 9.76
Trichy 141 15 10.64
Perambalur 67 12 17.91
Ariyalur 25 2 8.00
Dindigul 72 7 9.72
Madurai 156 23 14.74
Thanjavur 37 2 5.41
Coimbatore 93 7 7.53
1034 126 12.19

18. Thank you