Brief description of the three modalities of monitoring esophageal pH for the diagnosis of GERD.

1. 24 hour pH monitoring of the esophagus
Samir Haffar M.D.
Assistant Professor of Gastroenterology

2. pH monitoring of the esophagus
 Standard 24 hours pH monitoring
 Bravo capsule (tubeless capsule)
 Combined Multiple Intraluminal Impedance-pH
(MII-pH)

3.  Standard 24 hours pH monitoring of
esophagus

4. Sites of 24 hour pH Monitoring
• Single monitoring site: 5 cm above LES
• Multiple monitoring sites: 5 & 20 cm above LES
• One in the esophagus & one in proximal stomach
• One or more in esophagus & another in hyopharynx

5. 5 cm above LES

6. Why 5 cm above LES?
• Has been standard for many years
• Chosen to avoid catheter migration into stomach
• Moving by a 1 cm or two would not change results
• Moving it 10 cm above LES miss a number of patients
who are identified by the more distal location

7. Typical monitoring sites of 5 & 20 cm
above manometrically determined LES

8. Location of LES
• Manometric localization Reference method
• pH step-up method Sudden rise to pH > 4
• LES locator Prior to pH
• Fluoroscopic techniques Not accurate
• Endoscoic technique Not accurate

9. Ideal pH electrode
“No single probe meets all of these criteria”
• Small
• Firm enough
• Rapid response time between pH 7 to pH 1
• Minimally affected by temperature
• No hysteresis effect
• No drift during 24 hours
• Inexpensive
• Simple to calibrate or disposable

10. Which pH Electrode ?
Either can be used satisfactorily
Glass electrodes Antimony electrodes
40 – 50 studies 10 studies
Most linear response
Most rapid response Less response fidelity
Least recording drift
Large diameter Smaller
Siff bulky catheters More flexible
Expensive Less expensive

11. Indications of Esophageal pH Recording
• Normal endoscopic findings & reflux symptoms refractory to PPI
• Endoscopy-negative patient before surgical anti-reflux repair
• Patients suspected to have abnormal reflux after surgery
• Refractory reflux in pts with chest pain after cardiac evaluation
• Suspected ENT manifestations after failure of 4 weeks of PPI
• GERD in an adult onset non-allergic asthma
AGA Medical Position Statement. Gastroenterology 1996 ; 110 : 1981 - 96

12. No indications of esophageal pH recording
• Esophageal pH recording not indicated to detect or
verify reflux esophagitis (this is an endoscopic dg)
• Esophageal pH recording not indicated to evaluate
„„alkaline reflux‟‟
AGA Medical Position Statement. Gastroenterology 1996 ; 110 : 1981 - 1996

13. Why pH < 4?
• Defined early in development of the technology
• Its choice was based on:
- Marked difference from normal esophageal pH of 7
- Pepsinogen converted to pepsin at pH 4
- pH < 4 was one that tends to produce symptoms
• Some believe that drops in pH that do not reach level
of 4.0 still may represent reflux that these events
should also be used in calculations of indices

14. Composite scoring systems
Johnson & DeMeester is the most commonly used
Percentage of total time pH < 4
Percentage of upright time pH < 4
Percentage of supine time pH < 4
Number of reflux episodes
Number of reflux episodes >5 min
Longest reflux episode
DeMeester score
Normal < 14,72

15. Normal values of DeMeester’s score
50 healthy volunteers
DeMeester TR et al. Ann Surg 1976 ; 184 :459 – 470.

16. Normal 24 hours esophageal pH monitoring
Composite DeMeester score: 8.4
DeMeester normal < 14.72 (95th percentile)
Bremner CG et al. Esophageal disease & testing. Taylor & Francis Group, 2005.

17. Typical distal & proximal 24 hour esophageal pH

18. Nocturnal acid breakthrough
• Defined arbitrarily as intragastric pH < 4 for > 1 h
overnight during administration of PPI
• Occurs even on twice-daily therapy
• Common enough: rule rather than exception
• Not without controversy: little to do with reflux
• Addition of H2RAs at bedtime to PPI bid controls
NAB better than PPI therapy alone?

19. Nocturnal acid breakthrough
Gastric pH < 4 for at least 1 h during the night in patients
with persistent heartburn on standard dose PPIs twice daily
Combined gastric & esophageal 24 hr pH monitoring

20. Qualitative analysis
Symptom–reflux correlation
• Symptom index: Positive if ≥ 50%
• Symptom sensitivity index: Positive if > 10 %
• Symptom association probability Positive if > 95%
Determine relationship between heartburn episodes & acid
reflux events, regardless if pH test is normal or abnormal

21. Symptom index
Positive if 50 % or more
Not take into account overall number of reflux episodes

22. Symptom Sensitivity Index
Positive if 10 %
Not take into account overall number of symptoms

23. Symptom Association Probability Calculation
Positive if 95%
• Divides tracing into 2-min segments & looks at
whether a symptom & acid are present during each 2
minute segment
• The analysis uses contingency table analysis of 4
possible outcomes for each segment:
acid + symptom +
acid + symptom –
acid – symptom +
acid – symptom –

24. Overall amount of acid exposure & number of
reflux episodes are the focus of many studies
using ambulatory pH testing
Relationship between symptoms & esophageal
acid is equally (or perhaps more) important

25. 24 hour pH esophageal monitoring
On & off therapy
• Off therapy
Uncertainty about diagnosis of reflux
Mildest grades of esophagitis: redness - friability
Very short segments of BE
• On therapy
Patient who has failed a therapeutic trial
Patient has known reflux or highly likely to have reflux
pH probe in esophagus & another in stomach (NAB)

26. 24 hours pH monitoring & medications
• PPI should be stopped for 5 – 7 days
• Other medications should be stopped for 1 – 3 days
• Patient must not use antacids or other OTC
medications for duration of the study

27. Percentage of total time pH < 4
Normal values
• Off therapy
5 cm above LES
20 cm above LES 1 %
Periods of meals or acidic beverages excluded
• On therapy
5 cm above LES
20 cm above LES ?
* Based on 95% CI obtained in healthy subjects treated with omeprazole 40 mg qd
Kuo B et al. Am J Gastroenterol 1996 ; 91 : 1532 – 8.
4 – 5.5 %
1.6 – 4 %*

28. Abnormal acid exposure time in heartburn
Disease Percentage of total time pH < 4
Barrett‟s esophagus 93 %
* ENRD: Endoscpic Negative Reflux Disease
* *NERD: Non Erosive Reflux Disease
Erosive esophagitis 75 % (in one study)
ENRD*
NERD**
Functional heartburn
- SI > 50%
- SI < 50%
50 %
100 %
0 %
Hypersensitive esophagus
Non acid reflux or motor event
24 hr pH monitoring is not gold standard for diagnosis of GERD

29.  Bravo capsule

30. Bravo system (Medtronics)
Esophageal Probe
25 x 6 x 5.5 mm
Battery
pH
electrode
Suction
chamber
Radio
transmitter
Delivery system
Receiver
100 x 70 x 30 mm - 165 g

31. Advantages of Bravo capsule
• Better tolerance by patients
• Fixed position of the capsule (6 cm above SCJ*)
• Prolonged monitoring under more physiologic
conditions (48 hours)
* SCJ: squamocolumnar junction

32. Bravo normal values
50 asymptomatic volunteers
1st 24 h 2nd 24 h
Mean
(+ SD)
95th
percentile
Mean
(+ SD)
95th
percentile
% total time at pH < 4 1.79 (2.16 5.89 1.78 (1.78) 5.64
% upright time at pH < 4 2.45 (3.14) 7.81 2.54 (2.57) 7.46
% supine time at pH < 4 0.37 (1.18) 1.58 0.34 (1.28) 1.29
Number of reflux episodes 21.2 (18.5) 55.30 22.3 (19.8) 56.15
No of reflux episodes >5 min 0.62 (1.21) 3.55 0.75 (1.15) 3.00
Longest reflux episode 3.79 (4.31) 11.23 5.95 (4.52) 17.03
DeMeester score 6.02 (4.82) 15.93 5.95 (4.52) 15.48

33. Conventional pH vs Bravo capsule
Head to head comparison – 40 patients
Bruley des Varannes S. Gut 2005 ; 54 ; 1682 – 1686.

34. Bravo capsule
Causes of under-recording
• Data drop-up
• Short reflux event not recorded
• Reflux events appear shorter

35. Bravo capsule
Data drop-out
Malfunctions in the electronics or the receiver
Interpreted as artifact & not represented in final pH report
Improved by 7 cm antenna & use of fiberglass
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 307 – 318.

36. Bravo capsule
Short reflux events not recorded
Bruley des Varannes S. Gut 2005 ; 54 ; 1682 – 1686.

37. Bravo capsule
Reflux events appear shorter
Bruley des Varannes S. Gut 2005 ; 54 ; 1682 – 1686.

38. Trouble shooting in Bravo capsule
• Severe odynophagia & chest pain (5%)
Chest radiography to exclude perforation
Viscous lidocaine
Endoscopic removal if symptoms continue
• Capsule detachment
• Failure to disloge
Endoscopic removal similar to polypectomy

39. Bravo capsule
Classic early detachment (10% of patients)
Easily recognized during inspection of pH tracing
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 307 – 318.
Sudden prolonged drop in pH represents capsule in stomach
Sharp rise as capsule enters small intestine through pylorus

40. Endoscopic removal of Bravo capsule
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 307 – 318.

41.  Combined MII-pH
(MII-pH)

42. Principle of “MII”
• 2 steel rings separated by isolator
• Alternating-current generator to apply electrical PD
• Circuit closed through electrical charges (ions)
contained in structures surrounding the catheter
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 257 – 264.

43. Impedance scale
Refluxate: High conductivity & low impedance
Air: Low conductivity & high impedance
Bremner CG et al. Esophageal disease & testing.
Taylor & Francis Group, New York, 1st edition, 2005.

44. Advantages of MII
• Content of refluxate Liquid – Gas – Mixed
• Direction of bolus Anterograde – retrograde
• Height of refluxate
• pH characteristics Acid
(combined MII-pH) Weekly acid
Weekly alkaline
Acid re-reflux

45. Liquid bolus
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 257 – 264.
1) Initial drop Liquid enters impedance-measuring segment
2) Rise Bolus cleared from this segment
3) Overshoot Decreased luminal cross-section during contraction
4) Return to baseline

46. Air bolus
(Belch, Air swallow)
1) Rapid rise Presence of air bolus inside esophagus
2) Rapid decrease Air bolus clears from this segment
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 257 - 264.

47. Mixed air – liquid Bolus
1) Rapid Rise Air in front of the bolus
2) Rapid drop Liquid component of mixed bolus
3) Rise Liquid being cleared from this segment
4) Return to baseline
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 257 – 264.

48. Antegrade bolus movement (MII)
Observed during swallowing
Progression of impedance from proximal to distal
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 257 – 264.

49. Retrograde bolus movement (MII)
Observed in reflux
Progression of impedance from distal to proximal
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 257 – 264.

50. Combined MII
• MII used clinically only in combination
– With esophageal manometry (MII-EM)
– With pH (MII-pH)
• MII not considered as replacement for manometry &
pH techniques but as complementary procedure that
expands diagnostic potential of esophageal function
testing & reflux monitoring

51. Combined MII-pH probe
• Impedance orifices
3, 5, 7, 9, 15, & 17 cm from the tip
• pH orifice
5 cm from the tip
• MII-pH probe = pH probe
Do not change patient comfort
Bremner CG et al. Esophageal disease & testing.
Taylor & Francis Group, NY, 1st edition, 2005.

52. “Sleuth” monitor – Sandhill
“Sleuth” monitor attached to the catheter
& worn around a belt during the recording period

53. GERD classification by combined MII-pH
 Acid reflux
Reflux with drop of pH from above 4.0 to below 4.0
 Superimposed acid reflux (Acid re-reflux)
Acid reflux occurs while pH < 4.0
 Weakly acidic reflux
Reflux results in esophageal pH between 4.0 & 7.0
 Weakly alkaline reflux
Reflux with nadir esophageal pH does not drop < 7.0
Sifrim D et al. Gut 2004 ; 53 ; 1024 – 1031.

54. Acid reflux (MII-pH)
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 361 – 371.

55. GERD classification by combined MII-pH
 Acid reflux
Reflux with drop of pH from above 4.0 to below 4.0
 Superimposed acid reflux (Acid re-reflux)
Acid reflux occurs while pH < 4.0
 Weakly acidic reflux
Reflux results in esophageal pH between 4.0 & 7.0
 Weakly alkaline reflux
Reflux with nadir esophageal pH does not drop < 7.0
Sifrim D et al. Gut 2004 ; 53 ; 1024 – 1031.

56. Superimposed acid reflux (MII-pH)
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 361 – 371.

57. GERD classification by combined MII-pH
 Acid reflux
Reflux with drop of pH from above 4.0 to below 4.0
 Superimposed acid reflux (Acid re-reflux)
Acid reflux occurs while pH < 4.0
 Weakly acidic reflux
Reflux results in esophageal pH between 4.0 & 7.0
 Weakly alkaline reflux
Reflux with nadir esophageal pH does not drop < 7.0
Sifrim D et al. Gut 2004 ; 53 ; 1024 – 1031.

58. Weakly acidic reflux (MII-pH)
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 361 – 371.

59. GERD classification by combined MII-pH
 Acid reflux
Reflux with drop of pH from above 4.0 to below 4.0
 Superimposed acid reflux (Acid re-reflux)
Acid reflux occurs while pH < 4.0
 Weakly acidic reflux
Reflux results in esophageal pH between 4.0 & 7.0
 Weakly alkaline reflux
Reflux with nadir esophageal pH does not drop < 7.0
Sifrim D et al. Gut 2004 ; 53 ; 1024 – 1031.

60. Weakly alkaline reflux (MII-pH)
Gastrointest Endoscopy Clin N Am 2005 ; 15 : 361 – 371.

61. Recommendations for MII-pH monitoring
• Endoscopy-negative patients with heartburn or
regurgitation despite PPI & performed on PPI therapy
• Utility of impedance in refractory reflux patients with
chest pain or extraesophageal symptoms unproven
• Current interpretation relies on SI, SSI or SAP
• Therapeutic implications of abnormal test unproven
ACG Practice Guidelines: Esophageal reflux testing.
Am J Gastroenterol 2007 ; 102 : 668 – 685.

62. Advantages of 3 major types of pH testing
pH Tubeless Combined
MII-pH
Comfort _ + _
Monitoring > 24 h _ + _
Nonacid reflux _ _ +
Normal values + _ _
Proximal reflux + ? +
Gastric monitoring + ? +

63. Thank You