Dr. Nilesh Kate's document discusses oxygen transport. It begins by outlining the objectives of oxygen uptake in the lungs, transport in blood, and release in tissues. It then covers the introduction, uptake of oxygen by pulmonary blood due to the concentration gradient between the alveoli and arteries. Oxygen is transported in arterial blood both dissolved and bound to hemoglobin. The sigmoid shaped oxygen-hemoglobin dissociation curve allows for efficient loading and unloading of oxygen in tissues. Shifts in this curve are also discussed. Myoglobin assists with oxygen storage in muscle tissue.
2. OBJECTIVES.
Uptake of oxygen by pulmonary blood.
Transport of oxygen in arterial blood.
Release of oxygen in tissue.
Vehicle for transport of oxygen.
Saturday, December 10, 2016
3. INTRODUCTION
Transport of gases occurs along
the conc gradient.
Alveolar air PO2 : 104 mm Hg.
Arterial blood PO2 :95 mm Hg
Venous blood PO2: 40 mm Hg.
Tissue interstitial fluid: 40
mm Hg.
Saturday, December 10, 2016
4. UPTAKE OF OXYGEN BY
PULMONARY BLOOD.
Alveolar PO2 -104
mm Hg.
Arterial blood po2 40
mm Hg.
Conc gradient of 64
mm Hg, transport is
rapid.
Saturday, December 10, 2016
5. TRANSPORT OF OXYGEN IN
ARTERIAL BLOOD.
PO2 in pulmonary veins
104 mm Hg & in aorta
100 mm Hg.
Due to venous
admixture
Bronchial veins mix with
pulmonary capillaries
Coronary blood through
Thebesian Veins.
Saturday, December 10, 2016
6. TRANSPORT OF OXYGEN IN
ARTERIAL BLOOD.
Dissolved form
In combination with
haemoglobin.
Oxygen in Arterial blood –
20ml/100 ml, in venous
blood 15ml/100ml
So 5ml/100 ml
transported from lungs to
tissue.
Saturday, December 10, 2016
7. DISSOLVED FORM
Out of 5 ml transported 0.3 ml
in dissolved form & rest with
Hb.
As per Henry’s law dissolved gas
is proportional to Po2, so there
is NO LIMIT for this transport as
long as Po2 is high.
So dissolved O2 at high Po2
( Hyperbaric Oxygen) is used
for oxygenation in certain
poisoning (CO)where Hb is
denatured.
Saturday, December 10, 2016
8. IN COMBINATION WITH
HAEMOGLOBIN.
Oxygenation of haemoglobin
Oxygen carrying capacity of haemoglobin
O2-Hb dissociation curve.
Shifts in O2-Hb dissociation curve.
Concept of P50 & its significance.
O2-Hb dissociation curve of Hb-F
Effect of carbon monoxide on tranport of O2
Oxygen dissociation curve for myoglobin.
Saturday, December 10, 2016
9. OXYGENATION OF
HAEMOGLOBIN
Oxygen combine with
Hb to form loose &
reversible bond, this is
oxygenation & Not
Oxidation, convert
Deoxyhaemoglobin
into Oxyhaemoglobin.
Time taken 0.1 sec.
Saturday, December 10, 2016
10. OXYGEN CARRYING CAPACITY
OF HAEMOGLOBIN
1 gm of Hb bind with
1.34ml of O2
100 ml blood containing Hb
15gm% (15Χ 1.34) bind
20.1 ml of O2
But due to different shunts
decreases to 19.8 ml out of
which 0.3 ml in dissolved
form & 19.5 ml as
Oxyhemoglobin.
Saturday, December 10, 2016
11. DISADVANTAGE
Only Disadvantage
with this method is
there is Ceiling with
this transport due to Hb
saturation.
% Saturation is Avg
saturation of entire Hb
molecule in the blood.
Saturday, December 10, 2016
12. O2-HB DISSOCIATION CURVE.
When relation between
PO2 & % of Hb saturation
is plotted on graph we get
OXYGEN-HAEMOGLOBIN
DISSOCIATION CURVE.
It shows that % of Hb
saturation increases with
increase in PO2, but this
relationship is not linear
Saturday, December 10, 2016
13. SIGMOID SHAPED CURVE -
CAUSE
When 1 molecule of O2
combines with Hb ,
affinity of Hb increases
& so on
This is called Co-
operative binding
Kinetics.
Due to this O2-Hb
dissociation curve is
Sigmoid.
Saturday, December 10, 2016
14. SIGMOID SHAPED CURVE -
Advantage
Loading Zone – Related
to process of O2 uptake
in lungs.
Even Po2 falls below 60
mmHg saturation is still
90%, so loading zone
provide margin of safety.
Saturday, December 10, 2016
15. SIGMOID SHAPED CURVE -
Advantage
Unloading zone –
related to O2 delivery in
tissue, steep portion
below Po2 60mmHg.
So that relatively large
Oxygen is liberated at
minor fall in o2 tension.
( Buffering Effect)
Saturday, December 10, 2016
16. SHIFTS IN O2-HB
DISSOCIATION CURVE.
Shift to Right
-Decreased affinity of
Hb for O2.
Causes
PCO2 & pH – Increase in
Pco2 shift curve to
Right – Bohr’s effect.
Saturday, December 10, 2016
17. SHIFTS IN O2-HB
DISSOCIATION CURVE.
Temperature – increase
temperature is due to increase
heat production, increased
PCo2 due to rapid metabolism,
decreased PO2 due to rapid
consumption & decreased pH
due to lactic acid accumulation.
2,3-Diphosphoglycerate –
formed during Glycolysis via
EMBEDEN-MEYERHOF
pathway.
Saturday, December 10, 2016
18. SHIFTS IN O2-HB
DISSOCIATION CURVE.
Shift to left – increase
affinity of haemoglobin
for oxygen.
Causes –
Pco2
H + ion conc
Temperature
Fetal haemoglobin
Saturday, December 10, 2016
19. CONCEPT OF P50 & ITS
SIGNIFICANCE.
P50 – partial pressure of oxygen that produces 50%
of saturation
Normal P50 – 25-27 mmHg.
Significance – Hb affinity inversely proportional to
P50 value.
Decreased p50 – Hb gets saturated at low po2, i.e shift to
left
Increased p50 - Hb gets saturated at high po2, i.e shift to
right.
Saturday, December 10, 2016
20. O2-HB DISSOCIATION CURVE
OF HB-F
O2-Hb dissociation
curve shifted to Left
As its affinity for 2,3-
DPG is less than HbA
So its affinity to
combine with oxygen
is more than HbA.
Saturday, December 10, 2016
21. EFFECT OF CARBON MONOXIDE
ON TRANSPORT OF O2
CO has 200 more
affinity for Hb.
Combines with Hb
forms-
Carboxyhaemoglobin
Decreases functional Hb
conc.
And decreases O2
tension & O2 transport.
Saturday, December 10, 2016
22. OXYGEN DISSOCIATION CURVE
FOR MYOGLOBIN.
Present in Muscle specialised
for sustained contraction
Dissociation curve is
Rectangular Hyperbola – as
it takes O2 at low pressure
readily
It does not show Bohr’s effect
At po2 40 mm Hg it is 95%
saturated & Hb 75%
Act as temporary store house
of Oxygen
Saturday, December 10, 2016
23. RELEASE OF OXYGEN IN
TISSUE.
O2 release at Rest
O2 release during
exercise.
Saturday, December 10, 2016
24. O2 RELEASE AT REST
Oxygen Delivery – Normal o2 delivery is 1L/min
Oxygen Consumption – 5ml/100ml/min, so total
is 250 ml/min
Utilization Coefficient - % of O2 consumed out of
oxygen delivered to tissue.
250/1000 × 100 = 25%
Saturday, December 10, 2016
25. O2 RELEASE DURING
EXERCISE.
Depend on Severity of
exercise
Delivery of oxygen –
increased by
Increase blood flow due to
increase cardiac output, local
arteriole dilatation, increase
in open capillaries.
Increase RBC count due to
splenic contraction
Release of oxygen – by
right sift of curve.
Saturday, December 10, 2016
26. VEHICLE FOR TRANSPORT OF
OXYGEN.
At PO2 100 mm Hg
Whole blood – 19.8ml
Hb soln – 19.5 ml
plasma soln – 0.3 ml
At PO2 40 mm Hg
Whole blood – 5 ml
Hb soln – 1.5 ml
plasma soln – 0.18 ml
Saturday, December 10, 2016