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Aas mujtaba

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AAS

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Aas mujtaba

  1. 1. PRESENTATION ON: PRINCIPLE OF ATOMIC ABSORPTION SPECTROSCOPY(AAS) INTEGRAL UNIVERSITY,LUCKNOW Session: 2016-2017 Presented by: Under the guidence of: Mohd Muztaba M.Pharm Mr. Paramdeep Bagga Pharmacology
  2. 2.  Alan walsh in mid 1950s  One element can be estimated at time  Qualitative determination of trace metal in liquid  Detection of element 1 PPM  No sample preparation  No role of temperature
  3. 3. The technique was introduced in 1955 by Alan Walsh in Australia ( 1916 – 1998 ).
  4. 4.  When a solutin containing metallic species is introduced into flame,the vapour of the metal atom will be obtained some of the metal atom will be raised to a energy level to sufficient high to emitt the characterstic rediation of a metal  A phenomena that utilized in the familiar technique a emission flame photometry  But a large percentage of metal atom will remain in the non emitting ground state these ground state atom of a particular element are receptive of light radiation of there on specific resonsnce wavelength
  5. 5.  When a light of this wavelength is to pass through a flame having atom of the metallic species path of that light will be observed and the absorption will directly propotional to the density of atom in the flame  Once this value of absorption is known the concentration of metallic element can be known because the absorption is directly propotional to the density of atom in the flame  Mathemetically the total amount of light absorb may be given by expression
  6. 6.  V is the total amount of light absorb  e change on electron on mass  c speed out light  N total number of atom  f Oscillator strength  πe are constant
  7. 7. Sr.no Name of the substance Element assay Measured at nm 1 Ascorbic acid Fe 248.3nm 2 Cispalstin Ag 328nm 3 Glucagon Zn 213.9nm 4 Oxy prenol hydrochloride Pb 217nm 5 Parazosin hydrochloride Ni 232nm 6 Zinc Oxide Cd Pd 228.7nm 217nm
  8. 8.  Assay of total zinc in insulin suspension  Assay of palledeum in carbenicillin sodium  Assay of lead in petrol  Assay of pharmaceutical substance by AAS
  9. 9. I hope you liked My presentation. Thank you! 

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