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PRESENTATION ON:
PRINCIPLE OF ATOMIC ABSORPTION
SPECTROSCOPY(AAS)
INTEGRAL UNIVERSITY,LUCKNOW
Session: 2016-2017
Presented by: Under the guidence of:
Mohd Muztaba
M.Pharm Mr. Paramdeep Bagga
Pharmacology
 Alan walsh in mid 1950s
 One element can be estimated at time
 Qualitative determination of trace metal in liquid
 Detection of element 1 PPM
 No sample preparation
 No role of temperature
The technique was introduced in 1955 by Alan Walsh in
Australia ( 1916 – 1998 ).
 When a solutin containing metallic species is introduced into
flame,the vapour of the metal atom will be obtained some of
the metal atom will be raised to a energy level to sufficient
high to emitt the characterstic rediation of a metal
 A phenomena that utilized in the familiar technique a emission
flame photometry
 But a large percentage of metal atom will remain in the non
emitting ground state these ground state atom of a particular
element are receptive of light radiation of there on specific
resonsnce wavelength
 When a light of this wavelength is to pass through a flame
having atom of the metallic species path of that light will be
observed and the absorption will directly propotional to the
density of atom in the flame
 Once this value of absorption is known the concentration of
metallic element can be known because the absorption is
directly propotional to the density of atom in the flame
 Mathemetically the total amount of light absorb may be given
by expression
 V is the total amount of light absorb
 e change on electron on mass
 c speed out light
 N total number of atom
 f Oscillator strength
 πe are constant
Sr.no Name of the substance Element
assay
Measured at nm
1 Ascorbic acid Fe 248.3nm
2 Cispalstin Ag 328nm
3 Glucagon Zn 213.9nm
4 Oxy prenol hydrochloride Pb 217nm
5 Parazosin hydrochloride Ni 232nm
6 Zinc
Oxide
Cd
Pd
228.7nm
217nm
 Assay of total zinc in insulin suspension
 Assay of palledeum in carbenicillin sodium
 Assay of lead in petrol
 Assay of pharmaceutical substance by AAS
I hope you liked My
presentation.
Thank you! 

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Aas mujtaba

  • 1. PRESENTATION ON: PRINCIPLE OF ATOMIC ABSORPTION SPECTROSCOPY(AAS) INTEGRAL UNIVERSITY,LUCKNOW Session: 2016-2017 Presented by: Under the guidence of: Mohd Muztaba M.Pharm Mr. Paramdeep Bagga Pharmacology
  • 2.  Alan walsh in mid 1950s  One element can be estimated at time  Qualitative determination of trace metal in liquid  Detection of element 1 PPM  No sample preparation  No role of temperature
  • 3. The technique was introduced in 1955 by Alan Walsh in Australia ( 1916 – 1998 ).
  • 4.  When a solutin containing metallic species is introduced into flame,the vapour of the metal atom will be obtained some of the metal atom will be raised to a energy level to sufficient high to emitt the characterstic rediation of a metal  A phenomena that utilized in the familiar technique a emission flame photometry  But a large percentage of metal atom will remain in the non emitting ground state these ground state atom of a particular element are receptive of light radiation of there on specific resonsnce wavelength
  • 5.  When a light of this wavelength is to pass through a flame having atom of the metallic species path of that light will be observed and the absorption will directly propotional to the density of atom in the flame  Once this value of absorption is known the concentration of metallic element can be known because the absorption is directly propotional to the density of atom in the flame  Mathemetically the total amount of light absorb may be given by expression
  • 6.  V is the total amount of light absorb  e change on electron on mass  c speed out light  N total number of atom  f Oscillator strength  πe are constant
  • 7. Sr.no Name of the substance Element assay Measured at nm 1 Ascorbic acid Fe 248.3nm 2 Cispalstin Ag 328nm 3 Glucagon Zn 213.9nm 4 Oxy prenol hydrochloride Pb 217nm 5 Parazosin hydrochloride Ni 232nm 6 Zinc Oxide Cd Pd 228.7nm 217nm
  • 8.  Assay of total zinc in insulin suspension  Assay of palledeum in carbenicillin sodium  Assay of lead in petrol  Assay of pharmaceutical substance by AAS
  • 9. I hope you liked My presentation. Thank you! 