In this webinar, you will learn how to improve the speed & integrity of your results using MasterPlex QT with an emphasis on curve-fitting. Topics will include: 1) A close up look at the 4-PL and 5-PL non-linear regression models 2) Heteroscedasticity & Weighting 3) Ensuring the quality of your data with the QC Manager 4) How to use time-saving features such as templates & Best Fit 5) New features in MasterPlex QT 2010

1. Presenters: Robert Lynde Deputy Director Sales Operations & Strategic Alliances Manager Allen Liu Supervisor Technical Support & Project Management Webinar: Improving your results with MasterPlex QT July 29, 2010 This presentation accompanies the free webinar recording at: https://www1.gotomeeting.com/register/351125992

4. 4 Parameter Logistic (4PL) A = minimum asymptote D = maximum asymptote C = inflection point B = slope factor/steepness of curve < low standard OR lowest extrapolated value > high standard OR highest extrapolated value

5. Caution on Extrapolation tiny change in MFI = HUGE change in concentration

7. 5 Parameter Logistic (5PL)

9. Heteroscedasticity