Tb diagnosis

Laboratory Diagnosis of Tuberculosis The Emerging Trends Asst Prof Dr KAMRAN AFZAL FCPS (Microbiology)

Tuberculosis - The Captain of Death

Diagnostic Workup for TB ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Types of Specimens ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Specimen Collection ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

A - Direct Tests ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

1. Direct Smear Microscopy (DSM) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Light Microscopy ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Grading on Light Microscopy *record actual number of bacilli seen in 100 fields 100 neg No AFB in 100 oil immersion fields 100 Scanty* pos 1-9 AFB/100 oil immersion fields 100 1+ pos 10-99 AFB/100 oil immersion fields 50 2+ pos 1-10 AFB/ oil immersion field 20 3+ pos More than 10 AFB /oil immersion field No. of fields to be examined Grading Result If the slide has

Fluorescence Microscopy ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

2. Culture ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Processing of Sputum Specimens CONVENTIONAL or MOLECULAR METHODS CONVENTIONAL or MOLECULAR METHODS ID AST POSITIVE NEGATIVE CULTURE SOLID or LIQUID CONCENTRATION (Centrifuge) DIGESTION Decontamination Specimen Sputum Collection System SMEAR (Microscopy)

Colony Characteristics “ Rough, tough and buff” colonies of M. tuberculosis

Identification of M. tuberculosis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Culture ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Automated Culture Systems ,[object Object]

Antimicrobial Susceptibility Testing ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

3. Molecular Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Molecular Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

4. Antigen Detection ,[object Object],[object Object],[object Object],[object Object],[object Object]

5. Detection Of Lipids ,[object Object],[object Object],[object Object],[object Object],[object Object]

6. Animal Pathogenicity Testing ,[object Object],[object Object],[object Object],[object Object]

B - Indirect Tests ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

1. Tuberculin Skin Testing (TST) ,[object Object],[object Object],[object Object],[object Object]

TST: False Negatives/False Positives ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

2. TB Serology ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

3. Histopathology ,[object Object],[object Object],[object Object]

4. Haematological Findings ,[object Object],[object Object],[object Object]

Emerging Diagnostic Methods in TB ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object]

[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

[object Object],[object Object],[object Object],[object Object],Why we Fail to Diagnose TB?

Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Tb diagnosis

Recomendados

Recomendados

Más contenido relacionado

La actualidad más candente

La actualidad más candente (20)

Similar a Tb diagnosis

Similar a Tb diagnosis (20)

Más de Kamran Afzal, PhD.

Más de Kamran Afzal, PhD. (20)

Tb diagnosis