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Microscope
- 1. Introduction to the Microscope Care Parts Focusing
- 2. • An instrument that magnifies or makes tiny objects look bigger • Two kinds: – Simple – contains only one lens – Compound – two or more
- 3. 2 set lenses Choice of lens magnification
- 4. • This is a compound light microscope. • It is called this because it has two or more lenses (the eyepiece lenses and the objective Mechanism for enlarging lenses), and makes use an image of light to produce an image.
- 5. Maintains proper distance Eyepiece between lenses Magnification Body Tube Revolving Nosepiece Hold objective rotates to change magnification Arm Magnification Objectives Supports body tube Holds slide Stage Clips Stage Supports slide In place Coarse Focus Regulates Diaphragm Focuses image amount of light Fine Focus Light source Sharpens the image Reflects light towards eyepiece Base Supports microscope
- 6. the specimen the image the eye Each lens magnifies the image, increasing its overall size A lens is a bi-convex disk that bends light The bent rays produces an image The farther the light rays are bent, the larger the image appears
- 7. the specimen the image the eye As the light rays are spread Resolving power: A measure of farther apart, the clarity or the ability of a lens or optical system to form resolution of the image separate and decreases distinct images of two objects with small Light microscopes are limited in their angular separation. magnification by their resolving power!
- 9. the specimen the image the eye The image is always seen upside down and backwards from its actual position
- 10. The magnification • Place the Slide on the of the eyepiece is Microscope 10 times (10x) • Use Stage Clips to hold slide in place • Open the diaphragm (shutter) and switch on the lamp power • A microscope has several lens and each magnifies the specimen like a magnifying glass
- 11. •The two lenses are called: – Ey epi ec e – Ob jec tiv e •The eyepiece has a magnification of “10x” •The magnification of the objective lenses vary 400
- 12. Ocular lens Objective lenses 3 objective lens are 4X, 10X and 40X
- 13. •Always start with the low power objective (4X) •Always focus using the coarse knob, then the fine focus knob. •Centralise what you want further magnified •Switch to the 10X objective lens and if needed, to the 40X objective lens
- 14. • Follow steps to focus using low power • At high power, use only the fine focus knob! • Click the nosepiece to the longest objective • Do NOT use the coarse focusing knob as it will crack the slide. • Use the fine focus knob to bring the slide
- 15. • Do NOT remove the slide as it is very close to the objective lens. • To remove slide, switch to low-power or mid-power objective lens. What will you find?
- 16. Let’s Review! 9. Eyepiece 1. Body tube 2. Nosepiece 10. Arm 3. Low power 4. Medium power 11. Stage 5. High power 6. Stage clips 12. Course adjustment 7. Diaphragm 13. Fine adjustment 8. Light source 14. Base
- 17. • Always carry with 2 hands – one hand on the arm, the other on the base • Only use lens paper for cleaning • Do not force knobs • Always store covered • Keep desk clear of objects and cords
Notas del editor
- Students will have their microscopes out, for reference as we go through each of the steps. As an introduction, students will be asked what kinds of things they can do with this tool.
- Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down. Teacher will demonstrate how to store the microscope.
- Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down. Teacher will demonstrate how to store the microscope.
- This is the exact version of the microscope used in class. Students will be identifying the parts on the microscopes at their desks as we go along and what their functions are.
- Give students a slide from the “common things” set, each student will practice focusing and changing objectives.
- Have students exchange slides so they can look at different things, walk them through using the high power objective to focus slides. Emphasize not using the coarse objective during this process, as it will crack the slides.
- Teacher demonstrates how to hold the microscope, where the lens paper is located and how to use it. Students will be invited to turn the knobs and observe the stage as it moves up and down. Teacher will demonstrate how to store the microscope.