21. مائيينينرطو علىاملعتمدة فصل تقنيةATPS
Aqueous Two-Phase Systems (ATPS)
غير مائيين وسطين وجود على تعتمد استخالص تقنيةمزوجين
polymer–polymer and polymer–salt systems.
Most commonly used polymers include :
polyethylene glycol (PEG) and dextran
salts are phosphates, sulfates, and citrates
22. defined by their physicochemical properties such as
hydrophobicity, size, and net surface charge
العملية لتحسين اضداد اضافة يمكن.
23. based on cellular morphological and biophysical differences
such as mass, charge, size, density, shape, and rigidity. FFF is
achieved within an empty capillary channel by the combined
action of a transporting laminar flow of mobile phase and a
field that is applied perpendicularly to the flow
Field Flow Fractionation “FFF”
Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). Percoll is well suited for density gradient experiments because it possesses a low viscosity compared to alternatives, a low osmolarity and no toxicity towards cells and their constituents.
Percoll is a registered trademark o
طريقة ميكانيكة اسهل وارخص
The SVF consisted of a heterogeneous cell population, including circulating blood cells, fibroblasts, pericytes and endothelial cells as well as ‘‘pre-adipocytes”
or adipocyte progenitors
The final isolation step selected for the plastic adherent population within the SVF cells, which enriched for the ‘‘pre-adipocytes
التطبيقات العلاجية العلاج الجيني :This avoids the need to deliver high titers of the
viral vector directly to the patient. Before considering the
clinical application of ADAS cells as a gene carrier, stromal vascular fraction (SVF)additional in vitro and pre-clinical animal testing isnecessary.
طريقة ميكانيكة اسهل وارخص
The SVF consisted of a heterogeneous cell population, including circulating blood cells, fibroblasts, pericytes and endothelial cells as well as ‘‘pre-adipocytes”
or adipocyte progenitors
The final isolation step selected for the plastic adherent population within the SVF cells, which enriched for the ‘‘pre-adipocytes
التطبيقات العلاجية العلاج الجيني :This avoids the need to deliver high titers of the
viral vector directly to the patient. Before considering the
clinical application of ADAS cells as a gene carrier, stromal vascular fraction (SVF)additional in vitro and pre-clinical animal testing isnecessary.
The pelleted stromal cells are finally incubated with red blood cell lysis solution and
centrifuged one more time. This enzymatic procedure generates tissue fragments that
should be removed before cell plating through a 100–150 μm nylon meshenzymatic digestion is time consuming and expensive, especially when
applied to large volumes of tissue process was positive for mesenchymal surface markers such as
CD90 and CD105. They were also positive for CD34, which is reported only in adipose
tissue-derived mesenchymal cells