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Tissue Cultured
Pomegranate Plants Cultivation
Presented by,
Pomegranate
(Punica granatum)
PomumPomumPomumPomum:::: (Apple) granatusgranatusgranatusgranatus :(grainy)PomumPomumPomumPomum:::: (Apple) granatusgranatusgranatusgranatus :(grainy)
or
seeded apple.
Major Growing Countries
Pomegranate originated in Iran.Pomegranate originated in Iran.
India, Iran, China, USA and Turkey
IndiaIndiaIndiaIndia has occupied first positionfirst positionfirst positionfirst position in the world
with respect to pomegranate area (1.25 lakh ha.)
and production (8.21 lakh tonnes)
Presently Maharashtra,Maharashtra,Maharashtra,Maharashtra, Karnataka, AndhraPresently Maharashtra,Maharashtra,Maharashtra,Maharashtra, Karnataka, Andhra
Pradesh and Gujarat are the leading states in
Pomegranate cultivation.
India has low productivity (6.57 tonnes /ha)
compared to Spain (18.5 tonnes/ha)
Export of Pomegranate from India increasedExport of Pomegranate from India increased
5 times in 2009-10 over 2002-03
Bright future for export in years to come.
Edible portion (arils) of Pomegranate
fruit.
• 50 to 60 percent of total fruit weight
• Includes 65 to 80 per cent juice and 15 to 25
percent seeds; 20 to 30 percent peel forms.
• Citric acid predominantly found in juice.• Citric acid predominantly found in juice.
• Acidity below 0.6 percent.
• In Himalayan region, acidity may be 3.5 to 4.5
percent, used for anardana.
• The good source of antioxident.
Consumer performance
• Red colour skin
• Red colour arils (seeds)
• Red colour juice• Red colour juice
• Uniformity in size
• Round in shape
• Big size fruit
Climate
• Climate: Light loving plant. Best fruits in arid region
having long, hot and dry summer.
• Sub-tropical (hot season longer, cold is milder)to
tropical (high temp. and humid)
• Western Himalayan region:1600 to 3300 m above
mean sea level.
• Deccan Plateau region: 270 to 900 m above mean
sea level.
Soil
• Almost all types of soil. Especially best
growing in alluvial soil (fine, fertile soil), good
drainage.
• Saline tolerant crop
• Water soluble salts like sulphates, chlorides
and sodium have negative effect on root zone.
Variety: Bhagawa
• Good demand for domestic and export market.
• Bigger fruit size, sweet.
• Glossy red colour fruit skin, arils and juice.
• Mature fruits in 180 days after flowering.• Mature fruits in 180 days after flowering.
• Thick skin: Good for distant market.
• Present average rate: Rs. 40 to 100 per kg.
Orchard Establishment
Orchard Establishment
1. Long term investment
2. Well drained land with moderate slope 3-5
percent.
3. Assured electric supply for Irrigation facility.3. Assured electric supply for Irrigation facility.
4. Provision for fencing and wind brakes along
the boundary: Jamun, Casuarina (Suru), Silver
Oak.
Fencing
Planting system
• Variety: Bhagava
• Pit: 0.75 X 0.75 X 0.75 m
• Spacing: 4.5 X 3 m
• 740 plants per ha.• 740 plants per ha.
• Closer spacing may invite disease and pest
problem.
• Drench with 0.15 % Carbendazim + 0.2 %
Chlorpyriphos@2 to 4 lit/pit.
Use of Biofertilizers
• FYM:15 kg
• Vermi compost: 2 kg
• Neem cake: 1.5 kg
• Tricoderma: 25 gm.• Tricoderma: 25 gm.
• Phospate solubilising bacteria: 25 gm.
• Azatobactor: 25 gm.
• Kalisena (Aspergillus N.) and Josh
(Mycorrhiza): 25 gm each.
Planting
• Watering is done to allow soil to settle down.
• Use tissue cultured plants.
• Care: Earth ball should not break.
• Light irrigation is given after planting
immediately: drip irrigation.immediately: drip irrigation.
• Season: February -March; July-August
• Just after planting some support required for
keep plant straight. Use 1 m long bamboo/
wooden stick. Tie one or two places with coconut
strings.
Planting on raised bed and in furrow
Bamboo support
Training System
• Earlier training and pruning was not a
common practice.
• But fast growth of the industry, awareness
created.created.
• Single stem or Multi stem (3-4 stems allowed)
training.
• Within 2 years after planting develop proper
canopy.
Training System
Pruning stages
A) Young tree formation:
1. Pruning starts after 6-8 months to develop
structural framework.
2. 3-4 main limbs with lowest branch starting2. 3-4 main limbs with lowest branch starting
at 25-30 cm above the ground(single stem
training) or 3-4 stems (multi stem training).
3. Within 2 years after planting develop proper
canopy.
B) Maintenance pruning of bearing trees:
1. Pruning starts after third year.
2. Remove dead, diseased, broken and weak
branches.
3. Reduce tree height and width.3. Reduce tree height and width.
4. Improve light and air penetration.
5. Remove suckers from ground level.
Pruning technique
• A)A)A)A) ToppingToppingToppingTopping: Pruning is done to reduce height.
• In pre monsoon (May-June) or in winter(Dec-
January) after fruit harvesting.
• Applied organic manures and fertilizers after
topping.topping.
• B)B)B)B) SkirtingSkirtingSkirtingSkirting: Removal of lower tree branches.
This improves under tree access for irrigation,
weeding, fertilizers application.
New sprouts after pruning
Pruning tips:
• Regular part of tree management.
• Pruning minimises bending of branches and
staking.
• Sterilise pruning equipment : 3 % sodium
hypochloride.
• Sterilise pruning equipment : 3 % sodium
hypochloride.
• Heavy pruning should not be carried out in
hot weather; can result excessive growth.
• Avoid heavy pruning.
Flowering
• The flowers may be single or grouped in two or
three at the end of the branches.
• Generally cross pollination occurs by insect that
increases fruit set.
• Three seasons:
• A) Ambe bahar (January-February flowering)
• B) Mrig bahar (June-July flowering)• B) Mrig bahar (June-July flowering)
• C) Hasta bahar (Sept-Oct flowering )
• Ambe bahar is most preferred because of high
yield. We are recommending Hasta Bahar in
bacterial blight infected areas (best fruit quality).
Flowers
Flowers
• Male flower: are smaller bell shape or ovary
size.
• Intermediate flower: flower drops early or
mis-shaped fruits.
• Hermaphrodite flower: (male and female)• Hermaphrodite flower: (male and female)
well developed ovary. (60 to 70 % flowers in
TC)
• Flowers open in between 11 am to 2 pm at 37
to 38 degree C.
Bahar Treatment
• Only one crop should be taken in a year.
• Giving forced rest by giving stress before flower
initiation.
• Stopping water for 30 to 35 days (in light soil) to
2 months (in sandy loam soil) before defoliation.
• Stopping water for 30 to 35 days (in light soil) to
2 months (in sandy loam soil) before defoliation.
• In the last period of stress, defoliation is done
using ethrel 2to 2.5 ml/lit.
• Just after defoliation, (80 to 85 % leaf fall) light
pruning (15-20 cm) is done.
• Just after that light irrigation and recommended
dose of fertilizer is given.
• Then normal irrigation is recommended. Excess
irrigation avoided.
• The tree responds and new flush of flowering.
• Flowering starts within 30-60 days after first
irrigation.
• Flowering starts within 30-60 days after first
irrigation.
• First male flowers appears.
• Subsequently hermaphrodite flowers appears.
• Fruit development.
Manures and Fertilizers
• Most of the root system distributed in top
60cm of soil and within 1 m of the stem.
• Manures and fertilizers are applied in ring or• Manures and fertilizers are applied in ring or
small pits at 30-45 cm away from the main
stem below the tree canopy at 8-10 cm depth
and covered immediately.
Fertilizers application
Age of tree
(Yr)
FYM (Kg) Nitrogen (g) Phosphorus (g) Potash(g)
1 10 250 125 125
2 20 250 125 125
3 30 500 125 125
4 40 500 125 250
5 and above 45 625 250 250
1/3 recommended dose of manures and1/3rd recommended dose of manures and
fertilizers applied just after pruning in circular
trenches (8-10 cm depth) at 40-45 cm away
from the main stem; cover with soil and applied
irrigation.
• Remaining 2/3 recommended dose applied in
split doses after defoliation and pruning.
• First split (basal)dose consist of:
a) 1/3 rd dose of N and K
b) Full dose of P
c) Neem cake @ 1 kgc) Neem cake @ 1 kg
d) Vermicompost @1 kg
e) Phorate 20 g
f) Biofertilizers 25 gm each
Two split doses of N and K at 3-4 weeks
interval.
One month before fruit harvest , schedule two
drip irrigations applications of calciumdrip irrigations applications of calcium
nitrate 12.5 kg / ha/ application at 15 days
interval.
Water requirement
Age of the Tree-Yr (lit/day/tree)
Month 1 2 3 4 5 & above
Jan 2.7 8 20 30 40
Feb 2.8 10 28 40 55
Mar 2.9 11 30 45 57
April 3.2 12 35 50 60
May 3.5 13 35 50 65
June 2 7 21 30 40June 2 7 21 30 40
July 1.5 6 15 25 30
Aug 1.3 5 14 20 27
Sept 1.3 5 14 20 27
Oct 1.5 6 19 27 35
Nov 1.8 7 18 27 35
Dec 1.7 6 18 25 30
* Approx. Depends on soil, climate and weather conditions.
Drip irrigation system
Critical Stages for Irrigations
e.g. Ambe Bahar at 4th year (lit/day/tree)
I- New leaf initiation (30 days): Jan (10)
II- Blooming and fruit set (60 days): Feb, Mar (30)II- Blooming and fruit set (60 days): Feb, Mar (30)
III- Fruit development and maturity (120 days):
April, July (50)
IV- Harvesting (30 days): Aug. (25)
Inter-cropping
• Can be taken in rainy season
• Low growing vegetables like onion, cabbage
etc
• Pulses• Pulses
• Green manuring crops.
(in pre bearing orchards)
Inter-cropping
Weeding
• Can use black polythene mulch
• Can use organic mulches like paddy husk,
sugarcane trash, banana trash etc.
• Manual weeding is must.
Use of herbicides with proper care:
Manual weeding is must.
• Use of herbicides with proper care:
Pre-emergence-Simazine (triazine)
Post-emergence-Glyphosate
*(Avoid higher dose of herbicides)
Mulching
Fruit quality enhancement
• Thinning of fruits at appropriate stage.
• 60-80 fruits on fully grown tree.
• Good colour at ripening in Dec-Feb. preferred
for export.for export.
• Potassium application through foliar spray or
drip during fruit maturity.
• Yield: 15-20 tonnes / ha.
Important diseases and management
1. Bacterial blight (Telya): Oily spots can observe on
leaves, stem and fruits.
• Management: monthly spray
a) Streptocycline: 0.5 g/l
b) COC (copper oxy-chloride) : 2.5 g/l Orb) COC (copper oxy-chloride) : 2.5 g/l Or
a) Bronopol : 0.5 g/l
b) COC: 2.5 g/l
* Apply bordeaux paste(10 %) on cut portion of the tree.
Symptoms
2. Wilt: Caused by fungus, wilting of plant.
Management:
a) Drench with chlorpyriphos 20 EC (2.5 ml/l) and
carbendazim 50 WP(2 g/l)
OrOr
b) Propiconazole 25 EC (2 ml/l)@ 5 lit/tree
Repeat the drenching 3-4 times at 20 days interval.
Thank you…..
Presented by,
kisanconnect@gmail.com,
info@kisanconnect.com

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Tissue Culture Pomegranate Plants Cultivation in India

  • 1. Tissue Cultured Pomegranate Plants Cultivation Presented by,
  • 2. Pomegranate (Punica granatum) PomumPomumPomumPomum:::: (Apple) granatusgranatusgranatusgranatus :(grainy)PomumPomumPomumPomum:::: (Apple) granatusgranatusgranatusgranatus :(grainy) or seeded apple.
  • 3. Major Growing Countries Pomegranate originated in Iran.Pomegranate originated in Iran. India, Iran, China, USA and Turkey
  • 4. IndiaIndiaIndiaIndia has occupied first positionfirst positionfirst positionfirst position in the world with respect to pomegranate area (1.25 lakh ha.) and production (8.21 lakh tonnes) Presently Maharashtra,Maharashtra,Maharashtra,Maharashtra, Karnataka, AndhraPresently Maharashtra,Maharashtra,Maharashtra,Maharashtra, Karnataka, Andhra Pradesh and Gujarat are the leading states in Pomegranate cultivation.
  • 5. India has low productivity (6.57 tonnes /ha) compared to Spain (18.5 tonnes/ha) Export of Pomegranate from India increasedExport of Pomegranate from India increased 5 times in 2009-10 over 2002-03 Bright future for export in years to come.
  • 6. Edible portion (arils) of Pomegranate fruit. • 50 to 60 percent of total fruit weight • Includes 65 to 80 per cent juice and 15 to 25 percent seeds; 20 to 30 percent peel forms. • Citric acid predominantly found in juice.• Citric acid predominantly found in juice. • Acidity below 0.6 percent. • In Himalayan region, acidity may be 3.5 to 4.5 percent, used for anardana. • The good source of antioxident.
  • 7. Consumer performance • Red colour skin • Red colour arils (seeds) • Red colour juice• Red colour juice • Uniformity in size • Round in shape • Big size fruit
  • 8. Climate • Climate: Light loving plant. Best fruits in arid region having long, hot and dry summer. • Sub-tropical (hot season longer, cold is milder)to tropical (high temp. and humid) • Western Himalayan region:1600 to 3300 m above mean sea level. • Deccan Plateau region: 270 to 900 m above mean sea level.
  • 9. Soil • Almost all types of soil. Especially best growing in alluvial soil (fine, fertile soil), good drainage. • Saline tolerant crop • Water soluble salts like sulphates, chlorides and sodium have negative effect on root zone.
  • 10. Variety: Bhagawa • Good demand for domestic and export market. • Bigger fruit size, sweet. • Glossy red colour fruit skin, arils and juice. • Mature fruits in 180 days after flowering.• Mature fruits in 180 days after flowering. • Thick skin: Good for distant market. • Present average rate: Rs. 40 to 100 per kg.
  • 12. Orchard Establishment 1. Long term investment 2. Well drained land with moderate slope 3-5 percent. 3. Assured electric supply for Irrigation facility.3. Assured electric supply for Irrigation facility. 4. Provision for fencing and wind brakes along the boundary: Jamun, Casuarina (Suru), Silver Oak.
  • 14. Planting system • Variety: Bhagava • Pit: 0.75 X 0.75 X 0.75 m • Spacing: 4.5 X 3 m • 740 plants per ha.• 740 plants per ha. • Closer spacing may invite disease and pest problem. • Drench with 0.15 % Carbendazim + 0.2 % Chlorpyriphos@2 to 4 lit/pit.
  • 15. Use of Biofertilizers • FYM:15 kg • Vermi compost: 2 kg • Neem cake: 1.5 kg • Tricoderma: 25 gm.• Tricoderma: 25 gm. • Phospate solubilising bacteria: 25 gm. • Azatobactor: 25 gm. • Kalisena (Aspergillus N.) and Josh (Mycorrhiza): 25 gm each.
  • 16. Planting • Watering is done to allow soil to settle down. • Use tissue cultured plants. • Care: Earth ball should not break. • Light irrigation is given after planting immediately: drip irrigation.immediately: drip irrigation. • Season: February -March; July-August • Just after planting some support required for keep plant straight. Use 1 m long bamboo/ wooden stick. Tie one or two places with coconut strings.
  • 17. Planting on raised bed and in furrow
  • 19. Training System • Earlier training and pruning was not a common practice. • But fast growth of the industry, awareness created.created. • Single stem or Multi stem (3-4 stems allowed) training. • Within 2 years after planting develop proper canopy.
  • 21. Pruning stages A) Young tree formation: 1. Pruning starts after 6-8 months to develop structural framework. 2. 3-4 main limbs with lowest branch starting2. 3-4 main limbs with lowest branch starting at 25-30 cm above the ground(single stem training) or 3-4 stems (multi stem training). 3. Within 2 years after planting develop proper canopy.
  • 22. B) Maintenance pruning of bearing trees: 1. Pruning starts after third year. 2. Remove dead, diseased, broken and weak branches. 3. Reduce tree height and width.3. Reduce tree height and width. 4. Improve light and air penetration. 5. Remove suckers from ground level.
  • 23. Pruning technique • A)A)A)A) ToppingToppingToppingTopping: Pruning is done to reduce height. • In pre monsoon (May-June) or in winter(Dec- January) after fruit harvesting. • Applied organic manures and fertilizers after topping.topping. • B)B)B)B) SkirtingSkirtingSkirtingSkirting: Removal of lower tree branches. This improves under tree access for irrigation, weeding, fertilizers application.
  • 24. New sprouts after pruning
  • 25. Pruning tips: • Regular part of tree management. • Pruning minimises bending of branches and staking. • Sterilise pruning equipment : 3 % sodium hypochloride. • Sterilise pruning equipment : 3 % sodium hypochloride. • Heavy pruning should not be carried out in hot weather; can result excessive growth. • Avoid heavy pruning.
  • 26. Flowering • The flowers may be single or grouped in two or three at the end of the branches. • Generally cross pollination occurs by insect that increases fruit set. • Three seasons: • A) Ambe bahar (January-February flowering) • B) Mrig bahar (June-July flowering)• B) Mrig bahar (June-July flowering) • C) Hasta bahar (Sept-Oct flowering ) • Ambe bahar is most preferred because of high yield. We are recommending Hasta Bahar in bacterial blight infected areas (best fruit quality).
  • 28. Flowers • Male flower: are smaller bell shape or ovary size. • Intermediate flower: flower drops early or mis-shaped fruits. • Hermaphrodite flower: (male and female)• Hermaphrodite flower: (male and female) well developed ovary. (60 to 70 % flowers in TC) • Flowers open in between 11 am to 2 pm at 37 to 38 degree C.
  • 29. Bahar Treatment • Only one crop should be taken in a year. • Giving forced rest by giving stress before flower initiation. • Stopping water for 30 to 35 days (in light soil) to 2 months (in sandy loam soil) before defoliation. • Stopping water for 30 to 35 days (in light soil) to 2 months (in sandy loam soil) before defoliation. • In the last period of stress, defoliation is done using ethrel 2to 2.5 ml/lit. • Just after defoliation, (80 to 85 % leaf fall) light pruning (15-20 cm) is done.
  • 30. • Just after that light irrigation and recommended dose of fertilizer is given. • Then normal irrigation is recommended. Excess irrigation avoided. • The tree responds and new flush of flowering. • Flowering starts within 30-60 days after first irrigation. • Flowering starts within 30-60 days after first irrigation. • First male flowers appears. • Subsequently hermaphrodite flowers appears. • Fruit development.
  • 31. Manures and Fertilizers • Most of the root system distributed in top 60cm of soil and within 1 m of the stem. • Manures and fertilizers are applied in ring or• Manures and fertilizers are applied in ring or small pits at 30-45 cm away from the main stem below the tree canopy at 8-10 cm depth and covered immediately.
  • 33. Age of tree (Yr) FYM (Kg) Nitrogen (g) Phosphorus (g) Potash(g) 1 10 250 125 125 2 20 250 125 125 3 30 500 125 125 4 40 500 125 250 5 and above 45 625 250 250 1/3 recommended dose of manures and1/3rd recommended dose of manures and fertilizers applied just after pruning in circular trenches (8-10 cm depth) at 40-45 cm away from the main stem; cover with soil and applied irrigation.
  • 34. • Remaining 2/3 recommended dose applied in split doses after defoliation and pruning. • First split (basal)dose consist of: a) 1/3 rd dose of N and K b) Full dose of P c) Neem cake @ 1 kgc) Neem cake @ 1 kg d) Vermicompost @1 kg e) Phorate 20 g f) Biofertilizers 25 gm each
  • 35. Two split doses of N and K at 3-4 weeks interval. One month before fruit harvest , schedule two drip irrigations applications of calciumdrip irrigations applications of calcium nitrate 12.5 kg / ha/ application at 15 days interval.
  • 36. Water requirement Age of the Tree-Yr (lit/day/tree) Month 1 2 3 4 5 & above Jan 2.7 8 20 30 40 Feb 2.8 10 28 40 55 Mar 2.9 11 30 45 57 April 3.2 12 35 50 60 May 3.5 13 35 50 65 June 2 7 21 30 40June 2 7 21 30 40 July 1.5 6 15 25 30 Aug 1.3 5 14 20 27 Sept 1.3 5 14 20 27 Oct 1.5 6 19 27 35 Nov 1.8 7 18 27 35 Dec 1.7 6 18 25 30 * Approx. Depends on soil, climate and weather conditions.
  • 38. Critical Stages for Irrigations e.g. Ambe Bahar at 4th year (lit/day/tree) I- New leaf initiation (30 days): Jan (10) II- Blooming and fruit set (60 days): Feb, Mar (30)II- Blooming and fruit set (60 days): Feb, Mar (30) III- Fruit development and maturity (120 days): April, July (50) IV- Harvesting (30 days): Aug. (25)
  • 39. Inter-cropping • Can be taken in rainy season • Low growing vegetables like onion, cabbage etc • Pulses• Pulses • Green manuring crops. (in pre bearing orchards)
  • 41. Weeding • Can use black polythene mulch • Can use organic mulches like paddy husk, sugarcane trash, banana trash etc. • Manual weeding is must. Use of herbicides with proper care: Manual weeding is must. • Use of herbicides with proper care: Pre-emergence-Simazine (triazine) Post-emergence-Glyphosate *(Avoid higher dose of herbicides)
  • 43. Fruit quality enhancement • Thinning of fruits at appropriate stage. • 60-80 fruits on fully grown tree. • Good colour at ripening in Dec-Feb. preferred for export.for export. • Potassium application through foliar spray or drip during fruit maturity. • Yield: 15-20 tonnes / ha.
  • 44. Important diseases and management 1. Bacterial blight (Telya): Oily spots can observe on leaves, stem and fruits. • Management: monthly spray a) Streptocycline: 0.5 g/l b) COC (copper oxy-chloride) : 2.5 g/l Orb) COC (copper oxy-chloride) : 2.5 g/l Or a) Bronopol : 0.5 g/l b) COC: 2.5 g/l * Apply bordeaux paste(10 %) on cut portion of the tree.
  • 46. 2. Wilt: Caused by fungus, wilting of plant. Management: a) Drench with chlorpyriphos 20 EC (2.5 ml/l) and carbendazim 50 WP(2 g/l) OrOr b) Propiconazole 25 EC (2 ml/l)@ 5 lit/tree Repeat the drenching 3-4 times at 20 days interval.