CHEMICAL METHODS

1. INTRODUCTION TO MICROBIOLOGY AND
MYCOLOGY

2. DISINFECTION & STERILIZATION
 STERILIZATION
Process of
removing microbes
inclusive of
bacterial
endospores
 DISINFECTION
process of
removing microbes
except bacterial
endospores

3. DISINFECTIONDISINFECTION
CHEMICALS:disinfection or sterilization
DISINFECTANTS:
low level
intermediate level
high level

4. Physical method
Heat Radiation
Dry heat Moist heat(autoclave)
Prevaccum Gravity displacement
STERILIZATION

5. CHEMICAL METHODCHEMICAL METHOD

6. 2% GLUTARLDEHYDE
Sterilization Disinfection
8-10 Hrs used for :-
Pneumatic circuits. i.e.-
ventilator tubing O2 masks
ventury devices nebulizer
chamber
15-30 Mts used for dis
infecting endoscopes
Respiratory tubing's

7. ETHYLENE OXIDE(EO)
Used for polythene and plastic items
E.g.:- Ambu bag ,Baines circuit , Domes
, Biopsy Needle , Electric items ,
Rubber items

8. E.O. GAS
Pre-caution:-
• Scrupulous cleanliness and dryness
• Adequate aeration at least 12Hrs

9. VENTILATOR
 STERILE WATER FOR NEBULIZER & HUMIDIFIER
 PNEUMATIC CIRCUIT TO BE CHANGED WHEN
VISIBLY SOILED
 USE HEAT & MOISTURE EXCHANGE FILTER
 CONDENSATE IN TUBING TO BE DRAINED ONLY
TO THE WATER TRAPS
 HUMIDIFIER DOMES (AUTO CLAVED OR
STERILIZED BY 2% GLUTARALDEHYDE)

10. PNEUMATIC CIRCUITPNEUMATIC CIRCUIT
• WASHED UNDER RUNNING WATER
• IMMERSED IN 2% GLUTARALDEHYDE
FOR 8 TO 10Hrs

11. TRACHEOSTOMY CARETRACHEOSTOMY CARE
 TO BE GIVEN EVERY FOUR HOURS
 SKIN AROUND TRACHEOSTOMY TUBE CLEANED
WITH BETADINE
 INNER CANNULA OF METAL TRACHEOSTOMY
-CLEANED UNDER RUNNING WATER
-IMMERSED IN HYDROGEN PEROXIDE FOR
10MTS
- WASHED WITH WATER AND GAUZE WICK
-BOILED FOR 10 MINTS AND REPLACED ONCE
IT IS COOL

12. TRACHEAL SUCTIONING
USE DISPOSABLE SUCTION CATHETER
SUCTION JAR & TUBING CHANGED EVERY 24 Hrs
FILLED WITH 7% LYSOL FOR
1Hr
CLEANED WITH SOAP UNDER
RUING WATER

13. SUCTION TRAYS
o TO BE CHANGED EVERYDAY
o SUCTION CUPS
-CHANGED AFTER EACH USE
- IMMERSED IN 7% LYSOL FOR 1Hr
- WASHED WITH SOAP &WATER
- SENT FOR AUTOCLAVING

14. ICU – ISOLATION ROOM
SINGLE CLOSED CUBICLE
CASES – OPEN TB , ANTHRAX, MRSA etc…
UV LIGHT FOR DISINFECTION

15. FUMIGATION
NOT RECOMMENDEDNOT RECOMMENDED

16. ICU INSTRUMENTS
►SEPARATE:THERMOMETER, AMBU BAG,
BAINS CIRCUIT, STETHOSCOPE,
► BIOPSY NEEDLE - EO GAS STERILIZATION
►DISPOSABLE SUCTION CATHETER

17. ALL REUSABLE ITEMS
WASHED UNDER
RUNNING WATER
2% GLUTARALDEHYDE

18. DOUBLE AUTO
CLAVING1. HIV , HBV , HCV , MRSA INFECTTION
2. FOR RE- USABLE NON – SHARP
MATERILE
3. PLACED IN REDBAG

19. PERSONNEL
UNIVERSAL PRECAUTION
HAND WASHING GLOVES
SHARPSMASK

20. LINEN
o SOAKED IN 7% LYSOL hrs

21. SEPARATE AREA
(UTILITY ROOM)
 FOR EQUIPMENT DISINFECTION
PNEUMATIC CIRCUITS , O2MASK etc
 SUCTION JAR CLEANING AND
DISINFECTION
 CHEST DRAINAGE BOTTLES
 LINEN DISINFECTION

22. ROUTINE SURVEILLANCE IN ICU – ONCE
IN A MONTH