Technique of Taking Biopsy from Endometrium and Interpretation

1. Technique of Taking Biopsy from
Endometrium and Interpretation
Course no. : VSO 636
Course Title : Techniques in Obstetrics and Gynaecology
Presented By
Md. Abdullah Al Mubin
MS in Theriogenology
Dept. of Surgery and Obstetrics
FACULTY OF VETERINARY SCIENCE
BANGLADESH AGRICULTURAL UNIVERSITY

2. Talk Plan
• Introduction
• Objectives of taking biopsy
• Requirements
• Technique of taking endometrial biopsy
• Conclusion
• References

3. Introduction
An endometrial biopsy is a clinical procedure in which
a small piece of tissue from the lining of the uterus
(endometrium) is collected for examination under a
microscope. It helps clinician to make proper
diagnosis of disease.

4. Objectives of taking endometrium biopsy
1. To diagnosis of disease in the uterus
2. To know the condition of endometrium
3. To monitor fertility and infertility
4. To evaluate treatment response

5. Requirements
1. Uterine biopsy catheter
2. Al gloves
3. Antiseptic solution etc.
Fig. Uterine biopsy catheter

6. Technique of Taking Biopsy from Endometrium
1. Animal is controlled properly.
2. Left hand is introduced into the rectum.
3. Then, the uterine catheter is introduced into the
uterus by the manipulation of left hand.
4. The window of uterine biopsy catheter is kept open.
5. Then the uterus is pressed slightly on the window of
the uterine catheter and the windows is closed to cut
off some tissue from endometrium.
6. Then the catheter is withdrawn carefully.
7. Individual sample should be taken from each horn
and body of the uterus.
8. Finally these tissues are allowed to further
histological processes.

7. Interpretation
The bovine endometrium can be evaluated
histopathologically for-
1. Periglandular fibrosis
2. Cystic glandular changes
3. Cellular infiltration in the endometrium
a. Free cells- neutrophils, lymphocytes,
eosinophil, plasma cells, pigments bearing
macrophages etc.
b. Lymphoid nodule

8. Periglandular fibrosis
It is based on the visibility and clarity of
the fibroblast surrounding the
endometrial glands in the connective
tissue stroma . The connective tissue is
hypertrophied and distinctly modified.
The fibroblast become spindle –shape
and are closely packed parallel to each
other and slender nuclei.
Cystic glandular changes
Occur much less frequently than
periglandular fibrosis with wide
branched lamina and low cuboidal or
flattened epithelium.
Fig. Periglandular fibrosis
Fig. Cystic glandular changes

9. Cellular Infiltration of the Endometrium
Free cells: Functional cellular cyclic variation are
mostly marked in the surface epithelium and the
adjacent layer. These free cells are present only in
small numbers in normal cow.
Fig. Prominent cellular infiltration Fig. No cellular infiltration

10. Lymphoid nodules
Lymphoid nodules are masses of small round
cells(lymphocytic) with dense nuclei that often
have a fibrous connective tissue capsules around
them and are actually focal infiltration of
mononuclear cells. These are distributed at
random throughout the entire uterus within
otherwise normal endometrium stroma.

11. Conclusion
Biopsy is an effective method of diagnosis of
disease. Several studies noted that there is no
negative effect of biopsy sampling on the fertility of
animal. But the hygiene of biopsy catheter is very
important. Otherwise infection may be occurred.

12. References
Chapwanya, A., Meade, K.G., Narciandi, F., Stanley, P., et al.
(2010) Endometrial biopsy : a valuable clinical and research
tool in bovine reproduction. Theriogenology. [Online] 73 (7),
988–994. Available from:
doi:10.1016/j.theriogenology.2009.11.015.
Yilmaz, O. (2012) Uterine Microbiology and Histopathology in
Repeat Breeder Anatolian Water Buffaloes : An Abattoir Study
Makale Kodu ( Article Code ): KVFD-2012-6446 Repeat
Breeder Anadolu Nehir Mandalarında Uterus Mikrobiyolojisi ve
Histopatolojisi : Bir Mezbaha Çalışması. 18 (5), 791–798.

13. Thanks for Patience
Hearing